![]() ![]() Comparative transcriptome analysis showed upregulation of proteins involved in linear electron transfer (LET), CET, and downregulation of proteins involved in respiratory electron transfer (RET), giving hints to understand the increased levels of ATP and NADH. As a result, an approximately 30% increased intracellular ATP, 60% increased intracellular NADH concentrations and up to 60% increased biomass production with fourfold increased d-lactate production were achieved. To this end, we introduced an electron transport protein-encoding gene omcS into cyanobacterium Synechococcus elongatus UTEX 2973 and demonstrated that the introduced OmcS directs excess electrons from plastoquinone (PQ) to photosystem I (PSI) to stimulate cyclic electron transfer (CET). Here we propose a novel strategy to direct the electrons to generate more ATP from light reactions to provide sufficient NADH for lactate production. Photosynthetic electron is the sole energy source and excess electrons are wasted in the light reactions of photosynthesis. Thus, sufficient NADH supply is crucial to produce dehydrogenase-derived chemicals in cyanobacteria. ![]() However, most dehydrogenases prefer to utilize NADH as a cofactor. ![]() In phototrophic microbes, the NADPH generated from photosynthesis is the dominant form of reducing equivalent. An efficient supply of reducing equivalent is essential for chemicals production by engineered microbes. ![]()
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